目的 建立印度獐牙菜指纹图谱,探讨和验证双标线性校正法结合PDA辅助法用于色谱峰定性的可行性。方法 采用HPLC建立印度獐牙菜指纹图谱,使用相似度分析和二维聚类分析进行质量评价,并使用双标线性校正结合PDA辅助法进行色谱峰定性。结果 10批次印度獐牙菜药材选定10个色谱峰作为指纹图谱的共有峰,经化学计量学评价,可对其质量进行初步评价;双标线性校正法预测保留时间的精度优于相对保留时间法;双标线性校正法结合PDA辅助法可更好的对色谱峰进行定性。结论 与相对保留时间法相比,双标线性校正法预测保留时间的准确度更高,色谱柱的适用范围更广。双标线性校正结合PDA对色谱法进行辅助定性可扩大双标线性校正的使用范围。
Abstract
OBJECTIVE To establish the HPLC fingerprint of Swertia chirayita, and validate the feasibility of the double standard linear correction method using two reference substances with PDA assistance. METHODS The fingerprint of Swertia chirayita was established by HPLC, and similarity analysis and two-dimensional clustering analysis were used for quality evaluation. Linear calibration using two reference substances with PDA assistance was used for identification of chromatographic peak. RESULTS Ten chromatographic peaks were selected as common peaks in the fingerprint of 10 batches of Swertia chirayita. Preliminary evaluation of the quality was made by chemometrics evaluation. The precision of predicting retention time by linear calibration using two reference substances was superior to the relative retention time method. Linear calibration using two reference substances with PDA assistance could qualify the chromatographic peaks better. CONCLUSION Linear calibration using two reference substances is more accurate and suitable for more chromatographic columns than relative retention time method. PDA assistance can enlarge the scope of application of linear calibration using two reference substances.
关键词
印度獐牙菜 /
指纹图谱 /
化学计量学 /
双标线性校正法 /
PDA辅助色谱峰定性
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Key words
Swertia chirayita /
fingerprint /
chemometrics /
linear calibration using two reference substances /
PDA assistance
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中图分类号：
R917
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参考文献
[1] Drug standard of ministry of health of the People′s Republic of China. Tibetan medicine. vol Ⅰ(中华人民共和国卫生部药品标准,藏药第一册) .1995:28.
[2] SUN L, XU J M, TIAN J G, et al. Pharmacognostical study of Swertia chirayita . J Chin Med Mater (中药材), 2011,34(1):53-55.
[3] XIOA Y C, WEI L X, YANG H X, et al. Quality control of traditional Tibetan medicine Swertia chirayita. Chin Pharm J (中国药学杂志),2010,45(4):255-258.
[4] SUN L, TIAN J G, JIN H Y, et al. Determination of five active constituents in Swertia chirayita by HPLC .Chin Pharm Aff (中国药事),2010,24(7):687-689.
[5] YANG Y, LUO W Z, LIU X, et al. Determination and quality assessment of 10 ingredients gentiopicroside and sweroside and so on in Tibetan medicine Jia Di(Swertia chirayita). China J Chin Mater Med(中国中药杂志),2012,37(20):3141-3146.
[6] ZHONG G Y, YANG Y, FENG T T, et al. Evaluation of medicinal reasonableness and resource utilization of Dida (Zang Yin Cheng) commonly used in Tibetan medicine .China J Chin Mater Med(中国中药杂志),2012,37(17):2639-2645.
[7] TIAN C W, ZHANG T J, JIANG L H. Study on Swertia mussotii Franch. By HPLC fingerprint method combined with chemical pattern recognition approaches.Chin Pharm J (中国药学杂志),2013,48(18):1545-1549.
[8] PANG Y, SUN L, JIN H Y, et al. Discussion on application and technical requirements of substitute reference substance method for simultaneous determination of multi-components in traditional Chinese medicine.Chin J Pharm Anal(药物分析杂志), 2013,33(1):169-177.
[9] SUN L, JIN H Y, PANG Y, et al. Two reference substances for determination of multiple components(Ⅰ): Linear calibration using two reference substances for identification of chromatographic peaks.Chin J Pharm Anal(药物分析杂志), 2013,33(8): 1424-1430.
[10] SUN L, PANG Y, JIN H Y, et al. Two reference substances for determination of multiple components(Ⅱ): Influence of detection wavelength selection for quantitative analysis.Chin J Pharm Anal(药物分析杂志),2013,33(9):1578-1586.
[11] QIAO F, SUN L, JIN H Y, et al. Fingerprint analysis of Semen Strychni by chemometrics and linear calibration using two reference substances. Chin Pharm J (中国药学杂志),2014,49(10):886-889.
[12] WEI F, LI Q Y, MA L Y, et al. Simultaneous determination of 6 isoflavones in traditional Chinese medicine and functional foods with replacement method of chemical reference substance. Chin J Pharm Anal(药物分析杂志), 2009,29(5):725-730.
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脚注
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基金
国家十二五“重大新药创制”专项(2014ZX09304307-002);国家自然科学基金资助项目(81303214,81173506);国家食药总局进口药材标准增修订专项(JKYC2012-14)
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